Staining:Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER T4 DNA ligase New England Biolabs Cat#M0202S BsaI-HF New England Biolabs Cat#R3535 Kanamycin Fisher Scientific Cat#BP906-5 Zeocin Invivogen Cat#ant-zn-1 Pierce IgG Elution Buffer Thermo Fisher Cat#21004 brefeldin A Biolegend Cat#420601 Golgi Stop BD Biosciences Cat#554724 Intracellular Staining Permeabilization Wash Buffer Biolegend Cat#421002 Acetonitrile Fisher Scientific Cat#A9961 Critical commercial assays MagPlex Microspheres Luminex corporation Cat#MC10034-01; MC10062-01; MC10065-01; MC10052-01; MC10078-01; MC10075-01; MC10072-01 Agencourt CleanSEQ beads Beckman Coulter Cat#A29154 Bio-Gel P-2 size exclusion resin Bio-Rad Cat1504118 FluoSpheres TM NeutrAvidin TM -Labeled Microspheres, 1.0 μm, red fluorescent (580/605), 1% solids Invitrogen Cat#F8775 Empty 96-Well Macro SpinColumn with 7 μm frit, 1.1 mL Reservoir Plate Harvard Apparatus Cat#74-5649 Melon Gel IgG Purification Resins and Kits Thermo Scientific Cat#45212 Pierce TM Chromogenic Endotoxin Quant Kit Thermo Fisher Cat#A39552 FluoSpheres NeutrAvidin-Labeled Microspheres, 1.0 μm Molecular Probes Invitrogen Cat#F8776 UltraPure CD14 MicroBeads Miltenyi Biotech Cat#130-118-907 ELISA for Human IgG Mabtech Cat#3850-1AD-6 Experimental models: Cell lines Freestyle 293F Thermo Fisher R79007 THP-1 ATCC TIB-202; RRID:CVCL_0006 CD16.NK-92 ATCC PTA-6967; RRID:CVCL_V429 Experimental models: Organisms/strains Stellar competent cells Clontech Labs Cat#636766 M. tuberculosis: H37Rv P hsp60 -luciferase Andreu et al. 93 N/A Software and algorithms GlycanAssure Data Acquisition Software (version 1.0) Applied Biosystems Cat#A30467 FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com Prism (version 10.0) GraphPad https://www.graphpad.com/ scientific-software/prism/ Stata (version 16) StataCorp https://www.stata.com 16 Cell Reports 44, 116653, December 23, 2025 Blood samples were collected by venipuncture in sodium heparin tubes, plasma isolated by centrifugation, aliquoted, stored at − 80 ◦ C, and heat-inactivated (30 min, 55C) prior to use. .. Polyclonal IgG from patient samples was isolated by negative selection via Melon Gel resin (Thermo Fisher), concentrated by ultra-centrifugal filtration (Millipore), and quantified by ELISA (Mabtech) per manufacturers’ instructions. mAb An anti-ESAT-6 VH/k sequence (GenBank: LC189555.1) 96 was synthesized and cloned into a pUC19 vector with a human IgG1 Fc domain (WT or encoding the L234A/L235A [LALA] variant) as previously described.
Labeling:Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER T4 DNA ligase New England Biolabs Cat#M0202S BsaI-HF New England Biolabs Cat#R3535 Kanamycin Fisher Scientific Cat#BP906-5 Zeocin Invivogen Cat#ant-zn-1 Pierce IgG Elution Buffer Thermo Fisher Cat#21004 brefeldin A Biolegend Cat#420601 Golgi Stop BD Biosciences Cat#554724 Intracellular Staining Permeabilization Wash Buffer Biolegend Cat#421002 Acetonitrile Fisher Scientific Cat#A9961 Critical commercial assays MagPlex Microspheres Luminex corporation Cat#MC10034-01; MC10062-01; MC10065-01; MC10052-01; MC10078-01; MC10075-01; MC10072-01 Agencourt CleanSEQ beads Beckman Coulter Cat#A29154 Bio-Gel P-2 size exclusion resin Bio-Rad Cat1504118 FluoSpheres TM NeutrAvidin TM -Labeled Microspheres, 1.0 μm, red fluorescent (580/605), 1% solids Invitrogen Cat#F8775 Empty 96-Well Macro SpinColumn with 7 μm frit, 1.1 mL Reservoir Plate Harvard Apparatus Cat#74-5649 Melon Gel IgG Purification Resins and Kits Thermo Scientific Cat#45212 Pierce TM Chromogenic Endotoxin Quant Kit Thermo Fisher Cat#A39552 FluoSpheres NeutrAvidin-Labeled Microspheres, 1.0 μm Molecular Probes Invitrogen Cat#F8776 UltraPure CD14 MicroBeads Miltenyi Biotech Cat#130-118-907 ELISA for Human IgG Mabtech Cat#3850-1AD-6 Experimental models: Cell lines Freestyle 293F Thermo Fisher R79007 THP-1 ATCC TIB-202; RRID:CVCL_0006 CD16.NK-92 ATCC PTA-6967; RRID:CVCL_V429 Experimental models: Organisms/strains Stellar competent cells Clontech Labs Cat#636766 M. tuberculosis: H37Rv P hsp60 -luciferase Andreu et al. 93 N/A Software and algorithms GlycanAssure Data Acquisition Software (version 1.0) Applied Biosystems Cat#A30467 FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com Prism (version 10.0) GraphPad https://www.graphpad.com/ scientific-software/prism/ Stata (version 16) StataCorp https://www.stata.com 16 Cell Reports 44, 116653, December 23, 2025 Blood samples were collected by venipuncture in sodium heparin tubes, plasma isolated by centrifugation, aliquoted, stored at − 80 ◦ C, and heat-inactivated (30 min, 55C) prior to use. .. Polyclonal IgG from patient samples was isolated by negative selection via Melon Gel resin (Thermo Fisher), concentrated by ultra-centrifugal filtration (Millipore), and quantified by ELISA (Mabtech) per manufacturers’ instructions. mAb An anti-ESAT-6 VH/k sequence (GenBank: LC189555.1) 96 was synthesized and cloned into a pUC19 vector with a human IgG1 Fc domain (WT or encoding the L234A/L235A [LALA] variant) as previously described.
Purification:Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER T4 DNA ligase New England Biolabs Cat#M0202S BsaI-HF New England Biolabs Cat#R3535 Kanamycin Fisher Scientific Cat#BP906-5 Zeocin Invivogen Cat#ant-zn-1 Pierce IgG Elution Buffer Thermo Fisher Cat#21004 brefeldin A Biolegend Cat#420601 Golgi Stop BD Biosciences Cat#554724 Intracellular Staining Permeabilization Wash Buffer Biolegend Cat#421002 Acetonitrile Fisher Scientific Cat#A9961 Critical commercial assays MagPlex Microspheres Luminex corporation Cat#MC10034-01; MC10062-01; MC10065-01; MC10052-01; MC10078-01; MC10075-01; MC10072-01 Agencourt CleanSEQ beads Beckman Coulter Cat#A29154 Bio-Gel P-2 size exclusion resin Bio-Rad Cat1504118 FluoSpheres TM NeutrAvidin TM -Labeled Microspheres, 1.0 μm, red fluorescent (580/605), 1% solids Invitrogen Cat#F8775 Empty 96-Well Macro SpinColumn with 7 μm frit, 1.1 mL Reservoir Plate Harvard Apparatus Cat#74-5649 Melon Gel IgG Purification Resins and Kits Thermo Scientific Cat#45212 Pierce TM Chromogenic Endotoxin Quant Kit Thermo Fisher Cat#A39552 FluoSpheres NeutrAvidin-Labeled Microspheres, 1.0 μm Molecular Probes Invitrogen Cat#F8776 UltraPure CD14 MicroBeads Miltenyi Biotech Cat#130-118-907 ELISA for Human IgG Mabtech Cat#3850-1AD-6 Experimental models: Cell lines Freestyle 293F Thermo Fisher R79007 THP-1 ATCC TIB-202; RRID:CVCL_0006 CD16.NK-92 ATCC PTA-6967; RRID:CVCL_V429 Experimental models: Organisms/strains Stellar competent cells Clontech Labs Cat#636766 M. tuberculosis: H37Rv P hsp60 -luciferase Andreu et al. 93 N/A Software and algorithms GlycanAssure Data Acquisition Software (version 1.0) Applied Biosystems Cat#A30467 FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com Prism (version 10.0) GraphPad https://www.graphpad.com/ scientific-software/prism/ Stata (version 16) StataCorp https://www.stata.com 16 Cell Reports 44, 116653, December 23, 2025 Blood samples were collected by venipuncture in sodium heparin tubes, plasma isolated by centrifugation, aliquoted, stored at − 80 ◦ C, and heat-inactivated (30 min, 55C) prior to use. .. Polyclonal IgG from patient samples was isolated by negative selection via Melon Gel resin (Thermo Fisher), concentrated by ultra-centrifugal filtration (Millipore), and quantified by ELISA (Mabtech) per manufacturers’ instructions. mAb An anti-ESAT-6 VH/k sequence (GenBank: LC189555.1) 96 was synthesized and cloned into a pUC19 vector with a human IgG1 Fc domain (WT or encoding the L234A/L235A [LALA] variant) as previously described.
Enzyme-linked Immunosorbent Assay:Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER T4 DNA ligase New England Biolabs Cat#M0202S BsaI-HF New England Biolabs Cat#R3535 Kanamycin Fisher Scientific Cat#BP906-5 Zeocin Invivogen Cat#ant-zn-1 Pierce IgG Elution Buffer Thermo Fisher Cat#21004 brefeldin A Biolegend Cat#420601 Golgi Stop BD Biosciences Cat#554724 Intracellular Staining Permeabilization Wash Buffer Biolegend Cat#421002 Acetonitrile Fisher Scientific Cat#A9961 Critical commercial assays MagPlex Microspheres Luminex corporation Cat#MC10034-01; MC10062-01; MC10065-01; MC10052-01; MC10078-01; MC10075-01; MC10072-01 Agencourt CleanSEQ beads Beckman Coulter Cat#A29154 Bio-Gel P-2 size exclusion resin Bio-Rad Cat1504118 FluoSpheres TM NeutrAvidin TM -Labeled Microspheres, 1.0 μm, red fluorescent (580/605), 1% solids Invitrogen Cat#F8775 Empty 96-Well Macro SpinColumn with 7 μm frit, 1.1 mL Reservoir Plate Harvard Apparatus Cat#74-5649 Melon Gel IgG Purification Resins and Kits Thermo Scientific Cat#45212 Pierce TM Chromogenic Endotoxin Quant Kit Thermo Fisher Cat#A39552 FluoSpheres NeutrAvidin-Labeled Microspheres, 1.0 μm Molecular Probes Invitrogen Cat#F8776 UltraPure CD14 MicroBeads Miltenyi Biotech Cat#130-118-907 ELISA for Human IgG Mabtech Cat#3850-1AD-6 Experimental models: Cell lines Freestyle 293F Thermo Fisher R79007 THP-1 ATCC TIB-202; RRID:CVCL_0006 CD16.NK-92 ATCC PTA-6967; RRID:CVCL_V429 Experimental models: Organisms/strains Stellar competent cells Clontech Labs Cat#636766 M. tuberculosis: H37Rv P hsp60 -luciferase Andreu et al. 93 N/A Software and algorithms GlycanAssure Data Acquisition Software (version 1.0) Applied Biosystems Cat#A30467 FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com Prism (version 10.0) GraphPad https://www.graphpad.com/ scientific-software/prism/ Stata (version 16) StataCorp https://www.stata.com 16 Cell Reports 44, 116653, December 23, 2025 Blood samples were collected by venipuncture in sodium heparin tubes, plasma isolated by centrifugation, aliquoted, stored at − 80 ◦ C, and heat-inactivated (30 min, 55C) prior to use. .. Polyclonal IgG from patient samples was isolated by negative selection via Melon Gel resin (Thermo Fisher), concentrated by ultra-centrifugal filtration (Millipore), and quantified by ELISA (Mabtech) per manufacturers’ instructions. mAb An anti-ESAT-6 VH/k sequence (GenBank: LC189555.1) 96 was synthesized and cloned into a pUC19 vector with a human IgG1 Fc domain (WT or encoding the L234A/L235A [LALA] variant) as previously described.
Luciferase:Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER T4 DNA ligase New England Biolabs Cat#M0202S BsaI-HF New England Biolabs Cat#R3535 Kanamycin Fisher Scientific Cat#BP906-5 Zeocin Invivogen Cat#ant-zn-1 Pierce IgG Elution Buffer Thermo Fisher Cat#21004 brefeldin A Biolegend Cat#420601 Golgi Stop BD Biosciences Cat#554724 Intracellular Staining Permeabilization Wash Buffer Biolegend Cat#421002 Acetonitrile Fisher Scientific Cat#A9961 Critical commercial assays MagPlex Microspheres Luminex corporation Cat#MC10034-01; MC10062-01; MC10065-01; MC10052-01; MC10078-01; MC10075-01; MC10072-01 Agencourt CleanSEQ beads Beckman Coulter Cat#A29154 Bio-Gel P-2 size exclusion resin Bio-Rad Cat1504118 FluoSpheres TM NeutrAvidin TM -Labeled Microspheres, 1.0 μm, red fluorescent (580/605), 1% solids Invitrogen Cat#F8775 Empty 96-Well Macro SpinColumn with 7 μm frit, 1.1 mL Reservoir Plate Harvard Apparatus Cat#74-5649 Melon Gel IgG Purification Resins and Kits Thermo Scientific Cat#45212 Pierce TM Chromogenic Endotoxin Quant Kit Thermo Fisher Cat#A39552 FluoSpheres NeutrAvidin-Labeled Microspheres, 1.0 μm Molecular Probes Invitrogen Cat#F8776 UltraPure CD14 MicroBeads Miltenyi Biotech Cat#130-118-907 ELISA for Human IgG Mabtech Cat#3850-1AD-6 Experimental models: Cell lines Freestyle 293F Thermo Fisher R79007 THP-1 ATCC TIB-202; RRID:CVCL_0006 CD16.NK-92 ATCC PTA-6967; RRID:CVCL_V429 Experimental models: Organisms/strains Stellar competent cells Clontech Labs Cat#636766 M. tuberculosis: H37Rv P hsp60 -luciferase Andreu et al. 93 N/A Software and algorithms GlycanAssure Data Acquisition Software (version 1.0) Applied Biosystems Cat#A30467 FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com Prism (version 10.0) GraphPad https://www.graphpad.com/ scientific-software/prism/ Stata (version 16) StataCorp https://www.stata.com 16 Cell Reports 44, 116653, December 23, 2025 Blood samples were collected by venipuncture in sodium heparin tubes, plasma isolated by centrifugation, aliquoted, stored at − 80 ◦ C, and heat-inactivated (30 min, 55C) prior to use. .. Polyclonal IgG from patient samples was isolated by negative selection via Melon Gel resin (Thermo Fisher), concentrated by ultra-centrifugal filtration (Millipore), and quantified by ELISA (Mabtech) per manufacturers’ instructions. mAb An anti-ESAT-6 VH/k sequence (GenBank: LC189555.1) 96 was synthesized and cloned into a pUC19 vector with a human IgG1 Fc domain (WT or encoding the L234A/L235A [LALA] variant) as previously described.
Software:Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER T4 DNA ligase New England Biolabs Cat#M0202S BsaI-HF New England Biolabs Cat#R3535 Kanamycin Fisher Scientific Cat#BP906-5 Zeocin Invivogen Cat#ant-zn-1 Pierce IgG Elution Buffer Thermo Fisher Cat#21004 brefeldin A Biolegend Cat#420601 Golgi Stop BD Biosciences Cat#554724 Intracellular Staining Permeabilization Wash Buffer Biolegend Cat#421002 Acetonitrile Fisher Scientific Cat#A9961 Critical commercial assays MagPlex Microspheres Luminex corporation Cat#MC10034-01; MC10062-01; MC10065-01; MC10052-01; MC10078-01; MC10075-01; MC10072-01 Agencourt CleanSEQ beads Beckman Coulter Cat#A29154 Bio-Gel P-2 size exclusion resin Bio-Rad Cat1504118 FluoSpheres TM NeutrAvidin TM -Labeled Microspheres, 1.0 μm, red fluorescent (580/605), 1% solids Invitrogen Cat#F8775 Empty 96-Well Macro SpinColumn with 7 μm frit, 1.1 mL Reservoir Plate Harvard Apparatus Cat#74-5649 Melon Gel IgG Purification Resins and Kits Thermo Scientific Cat#45212 Pierce TM Chromogenic Endotoxin Quant Kit Thermo Fisher Cat#A39552 FluoSpheres NeutrAvidin-Labeled Microspheres, 1.0 μm Molecular Probes Invitrogen Cat#F8776 UltraPure CD14 MicroBeads Miltenyi Biotech Cat#130-118-907 ELISA for Human IgG Mabtech Cat#3850-1AD-6 Experimental models: Cell lines Freestyle 293F Thermo Fisher R79007 THP-1 ATCC TIB-202; RRID:CVCL_0006 CD16.NK-92 ATCC PTA-6967; RRID:CVCL_V429 Experimental models: Organisms/strains Stellar competent cells Clontech Labs Cat#636766 M. tuberculosis: H37Rv P hsp60 -luciferase Andreu et al. 93 N/A Software and algorithms GlycanAssure Data Acquisition Software (version 1.0) Applied Biosystems Cat#A30467 FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com Prism (version 10.0) GraphPad https://www.graphpad.com/ scientific-software/prism/ Stata (version 16) StataCorp https://www.stata.com 16 Cell Reports 44, 116653, December 23, 2025 Blood samples were collected by venipuncture in sodium heparin tubes, plasma isolated by centrifugation, aliquoted, stored at − 80 ◦ C, and heat-inactivated (30 min, 55C) prior to use. .. Polyclonal IgG from patient samples was isolated by negative selection via Melon Gel resin (Thermo Fisher), concentrated by ultra-centrifugal filtration (Millipore), and quantified by ELISA (Mabtech) per manufacturers’ instructions. mAb An anti-ESAT-6 VH/k sequence (GenBank: LC189555.1) 96 was synthesized and cloned into a pUC19 vector with a human IgG1 Fc domain (WT or encoding the L234A/L235A [LALA] variant) as previously described.
Clinical Proteomics:Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER T4 DNA ligase New England Biolabs Cat#M0202S BsaI-HF New England Biolabs Cat#R3535 Kanamycin Fisher Scientific Cat#BP906-5 Zeocin Invivogen Cat#ant-zn-1 Pierce IgG Elution Buffer Thermo Fisher Cat#21004 brefeldin A Biolegend Cat#420601 Golgi Stop BD Biosciences Cat#554724 Intracellular Staining Permeabilization Wash Buffer Biolegend Cat#421002 Acetonitrile Fisher Scientific Cat#A9961 Critical commercial assays MagPlex Microspheres Luminex corporation Cat#MC10034-01; MC10062-01; MC10065-01; MC10052-01; MC10078-01; MC10075-01; MC10072-01 Agencourt CleanSEQ beads Beckman Coulter Cat#A29154 Bio-Gel P-2 size exclusion resin Bio-Rad Cat1504118 FluoSpheres TM NeutrAvidin TM -Labeled Microspheres, 1.0 μm, red fluorescent (580/605), 1% solids Invitrogen Cat#F8775 Empty 96-Well Macro SpinColumn with 7 μm frit, 1.1 mL Reservoir Plate Harvard Apparatus Cat#74-5649 Melon Gel IgG Purification Resins and Kits Thermo Scientific Cat#45212 Pierce TM Chromogenic Endotoxin Quant Kit Thermo Fisher Cat#A39552 FluoSpheres NeutrAvidin-Labeled Microspheres, 1.0 μm Molecular Probes Invitrogen Cat#F8776 UltraPure CD14 MicroBeads Miltenyi Biotech Cat#130-118-907 ELISA for Human IgG Mabtech Cat#3850-1AD-6 Experimental models: Cell lines Freestyle 293F Thermo Fisher R79007 THP-1 ATCC TIB-202; RRID:CVCL_0006 CD16.NK-92 ATCC PTA-6967; RRID:CVCL_V429 Experimental models: Organisms/strains Stellar competent cells Clontech Labs Cat#636766 M. tuberculosis: H37Rv P hsp60 -luciferase Andreu et al. 93 N/A Software and algorithms GlycanAssure Data Acquisition Software (version 1.0) Applied Biosystems Cat#A30467 FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com Prism (version 10.0) GraphPad https://www.graphpad.com/ scientific-software/prism/ Stata (version 16) StataCorp https://www.stata.com 16 Cell Reports 44, 116653, December 23, 2025 Blood samples were collected by venipuncture in sodium heparin tubes, plasma isolated by centrifugation, aliquoted, stored at − 80 ◦ C, and heat-inactivated (30 min, 55C) prior to use. .. Polyclonal IgG from patient samples was isolated by negative selection via Melon Gel resin (Thermo Fisher), concentrated by ultra-centrifugal filtration (Millipore), and quantified by ELISA (Mabtech) per manufacturers’ instructions. mAb An anti-ESAT-6 VH/k sequence (GenBank: LC189555.1) 96 was synthesized and cloned into a pUC19 vector with a human IgG1 Fc domain (WT or encoding the L234A/L235A [LALA] variant) as previously described.
Isolation:Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER T4 DNA ligase New England Biolabs Cat#M0202S BsaI-HF New England Biolabs Cat#R3535 Kanamycin Fisher Scientific Cat#BP906-5 Zeocin Invivogen Cat#ant-zn-1 Pierce IgG Elution Buffer Thermo Fisher Cat#21004 brefeldin A Biolegend Cat#420601 Golgi Stop BD Biosciences Cat#554724 Intracellular Staining Permeabilization Wash Buffer Biolegend Cat#421002 Acetonitrile Fisher Scientific Cat#A9961 Critical commercial assays MagPlex Microspheres Luminex corporation Cat#MC10034-01; MC10062-01; MC10065-01; MC10052-01; MC10078-01; MC10075-01; MC10072-01 Agencourt CleanSEQ beads Beckman Coulter Cat#A29154 Bio-Gel P-2 size exclusion resin Bio-Rad Cat1504118 FluoSpheres TM NeutrAvidin TM -Labeled Microspheres, 1.0 μm, red fluorescent (580/605), 1% solids Invitrogen Cat#F8775 Empty 96-Well Macro SpinColumn with 7 μm frit, 1.1 mL Reservoir Plate Harvard Apparatus Cat#74-5649 Melon Gel IgG Purification Resins and Kits Thermo Scientific Cat#45212 Pierce TM Chromogenic Endotoxin Quant Kit Thermo Fisher Cat#A39552 FluoSpheres NeutrAvidin-Labeled Microspheres, 1.0 μm Molecular Probes Invitrogen Cat#F8776 UltraPure CD14 MicroBeads Miltenyi Biotech Cat#130-118-907 ELISA for Human IgG Mabtech Cat#3850-1AD-6 Experimental models: Cell lines Freestyle 293F Thermo Fisher R79007 THP-1 ATCC TIB-202; RRID:CVCL_0006 CD16.NK-92 ATCC PTA-6967; RRID:CVCL_V429 Experimental models: Organisms/strains Stellar competent cells Clontech Labs Cat#636766 M. tuberculosis: H37Rv P hsp60 -luciferase Andreu et al. 93 N/A Software and algorithms GlycanAssure Data Acquisition Software (version 1.0) Applied Biosystems Cat#A30467 FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com Prism (version 10.0) GraphPad https://www.graphpad.com/ scientific-software/prism/ Stata (version 16) StataCorp https://www.stata.com 16 Cell Reports 44, 116653, December 23, 2025 Blood samples were collected by venipuncture in sodium heparin tubes, plasma isolated by centrifugation, aliquoted, stored at − 80 ◦ C, and heat-inactivated (30 min, 55C) prior to use. .. Polyclonal IgG from patient samples was isolated by negative selection via Melon Gel resin (Thermo Fisher), concentrated by ultra-centrifugal filtration (Millipore), and quantified by ELISA (Mabtech) per manufacturers’ instructions. mAb An anti-ESAT-6 VH/k sequence (GenBank: LC189555.1) 96 was synthesized and cloned into a pUC19 vector with a human IgG1 Fc domain (WT or encoding the L234A/L235A [LALA] variant) as previously described.
Centrifugation:Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER T4 DNA ligase New England Biolabs Cat#M0202S BsaI-HF New England Biolabs Cat#R3535 Kanamycin Fisher Scientific Cat#BP906-5 Zeocin Invivogen Cat#ant-zn-1 Pierce IgG Elution Buffer Thermo Fisher Cat#21004 brefeldin A Biolegend Cat#420601 Golgi Stop BD Biosciences Cat#554724 Intracellular Staining Permeabilization Wash Buffer Biolegend Cat#421002 Acetonitrile Fisher Scientific Cat#A9961 Critical commercial assays MagPlex Microspheres Luminex corporation Cat#MC10034-01; MC10062-01; MC10065-01; MC10052-01; MC10078-01; MC10075-01; MC10072-01 Agencourt CleanSEQ beads Beckman Coulter Cat#A29154 Bio-Gel P-2 size exclusion resin Bio-Rad Cat1504118 FluoSpheres TM NeutrAvidin TM -Labeled Microspheres, 1.0 μm, red fluorescent (580/605), 1% solids Invitrogen Cat#F8775 Empty 96-Well Macro SpinColumn with 7 μm frit, 1.1 mL Reservoir Plate Harvard Apparatus Cat#74-5649 Melon Gel IgG Purification Resins and Kits Thermo Scientific Cat#45212 Pierce TM Chromogenic Endotoxin Quant Kit Thermo Fisher Cat#A39552 FluoSpheres NeutrAvidin-Labeled Microspheres, 1.0 μm Molecular Probes Invitrogen Cat#F8776 UltraPure CD14 MicroBeads Miltenyi Biotech Cat#130-118-907 ELISA for Human IgG Mabtech Cat#3850-1AD-6 Experimental models: Cell lines Freestyle 293F Thermo Fisher R79007 THP-1 ATCC TIB-202; RRID:CVCL_0006 CD16.NK-92 ATCC PTA-6967; RRID:CVCL_V429 Experimental models: Organisms/strains Stellar competent cells Clontech Labs Cat#636766 M. tuberculosis: H37Rv P hsp60 -luciferase Andreu et al. 93 N/A Software and algorithms GlycanAssure Data Acquisition Software (version 1.0) Applied Biosystems Cat#A30467 FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com Prism (version 10.0) GraphPad https://www.graphpad.com/ scientific-software/prism/ Stata (version 16) StataCorp https://www.stata.com 16 Cell Reports 44, 116653, December 23, 2025 Blood samples were collected by venipuncture in sodium heparin tubes, plasma isolated by centrifugation, aliquoted, stored at − 80 ◦ C, and heat-inactivated (30 min, 55C) prior to use. .. Polyclonal IgG from patient samples was isolated by negative selection via Melon Gel resin (Thermo Fisher), concentrated by ultra-centrifugal filtration (Millipore), and quantified by ELISA (Mabtech) per manufacturers’ instructions. mAb An anti-ESAT-6 VH/k sequence (GenBank: LC189555.1) 96 was synthesized and cloned into a pUC19 vector with a human IgG1 Fc domain (WT or encoding the L234A/L235A [LALA] variant) as previously described.
other:Article Title: BNT162b2-induced neutralizing and non-neutralizing antibody functions against SARS-CoV-2 diminish with age.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anthrax Lethal Factor BEI Resources NR-28544 Anthrax Edema Factor BEI Resources NR-36210 Respiratory Syncytial Virus G protein from strain B1 BEI Resources NR-31098 Respiratory Syncytial Virus F protein from strain B1 BEI Resources NR-31097 Respiratory Syncytial Virus G protein from strain A2 BEI Resources NR-31096 Recombinant FcgRIIIa/CD16a R&D Systems Cat#4325-FC Recombinant FcgRIIa/CD32a R&D Systems Cat#9595-CD Recombinant FcgRIIb/CD32b R&D Systems Cat#1875-CD PE/R-Phycoerythrin Conjugation Kit Abcam Cat# ab102918 EZ-LinkTM Sulfo-NHS-LC-Biotin Thermo Scientific Cat#21335 Streptavidin Magnetic Beads New England Biolabs Cat#S1420S PureProteomeTM Protein G Magnetic Bead System Millipore Cat#LSKMAGG10 PNGase F New England Biolabs Cat#P0704S 8-aminoinopyrene-1,3,6-trisulfonic acid ThermoFisher Cat#A6257 Guinea pig complement Cedarlane Cat#CL4051 brefeldin A Biolegend Cat#420601 Golgi Stop BD Biosciences Cat#554724 Intracellular Staining Permeabilization Wash Buffer Biolegend Cat#421002 Acetonitrile Fisher Scientific Cat#A9961 Experimental models: Cell lines Vero E6 ATCC CRL-1586; RRID:CVCL_0574 THP-1 ATCC TIB-202; RRID:CVCL_0006 CD16.NK-92 ATCC PTA-6967; RRID:CVCL_V429 Software and algorithms Viridot (version 1.0) Katzelnick et al. (2018) https://github.com/leahkatzelnick/Viridot FRNT50 calculator (version 1.0) Bates et al. (2021b) https://github.com/tbates0/ FRNT50_calculator/tree/v1.0 R (version 4.2.1) R Project https://www.r-project.org/ Python (version 3.8.13) Python Software Foundation https://www.python.org/ GlycanAssure Data Acquisition Software (version 1.0) Applied Biosystems Cat#A30467 FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com Prism (version 9.0) GraphPad https://www.graphpad.com/ scientific-software/prism/ Stata (version 17) StataCorp https://www.stata.com Other MagPlex Microspheres Luminex corporation Cat# MC10043-01; MC10034-01; MC10037-01; MC10065-01 Agencourt CleanSEQ beads Beckman Coulter Cat#A29154 Bio-Gel P-2 size exclusion resin Bio-Rad Cat#1504118 FluoSpheresTM NeutrAvidinTM-Labeled Microspheres, 1.0 mm, red fluorescent (580/ 605), 1% solids Invitrogen Cat#F8775 Empty 96-Well Macro SpinColumn with 7 mm frit, 1.1 mL Reservoir Plate Harvard Apparatus Cat#74-5649 e2 Cell Reports 41, 111544, October 25, 2022
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